Selected scientific publications since 1996

Azhagiri, A. and Maliga, P. (2007) Exceptional paternal inheritance of plastids in Arabidopsis suggests that low frequency leakage of plastid via pollen may be universal in plants. Plant J. in press

Svab, Z. and Maliga, P. (2007) Exceptional transmission of plastids and mitochondria from the transplastomic pollen parent and its impact on transgene containment. Proc. Natl. Acad. Sci. USA 104: 7003-7008,

Azhagiri, A., Maliga, P. (2007) DNA markers define plastid haplotypes in Arabidopsis thaliana. Curr. Genet. 51: 269-275

Kittiwongwattana, C., Lutz K., Clark, M. and Maliga, P. (2007) Plastid marker gene excision by the phiC31 phage site-specific recombinase. Plant Mol. Biol. 64: 137-143

Lutz, K.A. and Maliga P. (2007) Construction of marker-free transplastomic plants. Curr. Opin. Biotechnol. 18: 107-114.

Lutz, K. and Maliga, P. (2007) Transformation of the plastid genome to study RNA editing. Methods Enzymol. 424: 501-518

Lutz, K.A., Bosacchi, M.H. and Maliga, P. (2006) Plastid marker gene excision by transiently expressed CRE recombinase. Plant J.  45: 4437-456.

Tungsuchat, T., Kuroda, H., Narangajavana, J. and Maliga, P. (2006) Gene activation in plastids by the CRE site-specific recombinase. Plant Mol. Biol. 61(4-5): 711-718.

Lutz, K.A., Svab, Z. and Maliga, P. (2006) Construction of marker-free transplastomic tobacco using the CRE-loxP site-specific recombination system. Nature Protocols. 1(2): 900-910.

Chakrabarti, S.K., Lutz, K.A., Lertwirijawong, B., Svab, Z. and Maliga, P. (2006) Expression of the cry9Aa2 Bt gene in tobacco chloroplasts confers extreme resistance to potato tuber moth. Transgenic Research 15: 485-492.

Maliga, P. (2005) New vectors and marker excision systems mark progress in engineering the plastid genome of higher plants. Photochem. Photobiol. Sci. 4: 971-976.

Maliga, P. (2004) Plastid transformation in higher plants. Annu. Rev. Plant Biol. 55: 289-313.

Lutz, K., Corneille, S., Azhagiri, A., Svab Z. and Maliga, P. (2004) Novel approach to plastid transformation utilizes the phiC31 phage site-specific integrase. Plant J. 37: 906-913.

Suzuki, J.Y., Ytterberg, A.J., Beardslee, T.A., Allison, L.A., van Wijk, K.J. and Maliga, P. (2004) Affinity purification of the tobacco plastid RNA polymerase and in vitro reconstitution of the holoenzyme. Plant J. 40: 164-172.

Maliga, P. (2003) Progress towards commercialization of plastid transformation technology. Trends in Biotechnology, 21:20-28.

Suzuki, J., Sriraman, P. Svab, Z. and Maliga, P. (2003) Unique architecture of the plastid rRNA operon recognized by the multisubunit RNA polymerase in tobacco and other higher plants. Plant Cell 15: 195-215.

Skarjinskaia, M., Svab, Z., Kuroda, H. and Maliga P. (2003) Plastid transformation in Lesquerella fendleri, an oilseed brassicacea. Transgenic Res. 12: 115-122.

Tregoning, J., Nixon, Kuroda, H.,  Svab, Z., Clare, S., Bowe, F., Fairweather, N., Y. Ytterberg, K.J. vanWijk, Dougan, G. and Maliga, P. (2003) Expression of tetanus toxin fragment C in tobacco chloroplasts. Nucleic Acids Res. 31: 1174-1179.

Corneille, S., Lutz, K.A., Azhagiri, A.K. and Maliga, P. (2003) Identification of functional lox sites in the plastid genome. Plant J. 35: 753-762.

Kuroda, H. and Maliga, P. (2003) The plastid clpP1 gene is essential for plant development. Nature 425: 86-89.

Kuroda, H. and Maliga, P. (2002) Over-expression of the clpP 5'-UTR in a chimeric context causes a mutant phenotype suggesting competition for a clpP-specific RNA maturation factor in tobacco chloroplasts. Plant Physiol. 129: 1600-1606.

Maliga, Pal (2002) Engineering the plastid genome of higher plants. Curr. Opin. Plant Biol. 5: 164-172.

Kuroda, H. and Maliga, P. (2001) Sequences downstream of the translation initiation codon are important determinants of translation efficiency in chloroplasts. Plant Physiol. 125: 430-436.

Kuroda, H. and Maliga, P. (2001) Complementarity of the 16S rRNA penultimate stem with sequences downstream of the AUG destabilizes the plastid mRNAs. Nucleic Acids Res. 29: 970-975.

Lutz, K.A., Knapp, J.E. and Maliga, P. (2001) Expression of bar in the plastid genome confers herbicide resistance. Plant Physiol. 125: 1585-1590.

Corneille, S., Lutz, K., Svab, Z. and Maliga, P. (2001) Efficient elimination of selectable marker genes from the plastid gnome by the CRE-lox site-specific recombination system. Plant J. 27: 171-178.

Liere, K. and Maliga, P. (2001) Plastid RNA polymerases in higher plants. In: Regulation of Photosynthesis, E.M. Aro and B. Anderson (eds.) Kluwer Academic Publishers, pp. 29-49.

Lutz, K. and Maliga, P. (2001) Lack of conservation of editing sites in mRNA that encode subunits of the ND(P)H dehydrogenase complex in plastids and mitochondria of Arabidopsis thaliana. Curr. Genet. 40:214-219.

Corneille, S., Lutz, K. and Maliga, P. (2000) Conservation of editing between rice and maize plastids: Are most editing sites dispensable? Mol. Gen. Gent. 264: 419-424.

Liere, K., and Maliga, P. (1999) In vitro characterization of the tobacco rpoB promoter reveals conserved promoter architecture for the phage-type plastid and mitochondrial RNA polymerases. EMBO J. 18: 249-257.

Kanevski, I., Maliga, P., Rhoades, D.F., and Gutteridge, S. (1999) Plastome engineering of ribulose-1,5-bispohosphate carboxylase/oxygenase in tobacco to form a sunflower large subunit and a tobacco small subunit hybrid. Plant Physiol. 119: 1-9.

Khan, M.S. and Maliga, P. (1999) Fluorescent antibiotic resistance marker for tracking plastid transformation in higher plants. Nat. Biotechnol.17: 910-915.

Maliga, P. (1998) Two plastid RNA polymerases of higher plants: an evolving story. Trends in Plant Science 3: 4-6.

Burrows, P., Sazanov, L.A., Svab, Z. Maliga, P. and Nixon, P.J. (1998) Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes. EMBO J. 17: 868-876.

Silhavy, D. and Maliga, P. (1998) Mapping of promoters for the nucleus-encoded plastid RNA polymerase (NEP) in the iojap maize mutant. Curr. Genet. 33: 340-344.

Silhavy, D. and Maliga, P. (1998) Plastid promoter utilization in rice embryogenic cell culture. Curr. Genet. 34: 67-70.

Serino, G. and Maliga, P. (1998) RNA polymerase subunits encoded by the plastid rpo genes are not shared with the nucleus-encoded plastid enzyme. Plant Physiol. 117: 1165-1170.

Sriraman, P., Silhavy, D., Maliga, P. (1998) Transcription from heterologous ribosomal RNA operon promoters in chloroplasts reveals requirement for specific activating factors. Plant Physiol. 117: 1495-1499.

Shiina, T., Allison, L.A., and Maliga, P. (1998) rbcL transcript levels in tobacco plastids are independent of light: reduced dark transcription rate is compensated by increased mRNA stability. Plant Cell 10: 1713-1722.

Maliga, P., and Nixon, P.J. (1998) Judging the homoplastomic state of plastid transformants. Trends Plant Sci. 3: 376-377.

Sikdar, S.R., Serino, G., Chaudhuri, S., and Maliga, P. (1998) Plastid transformation in Arabidopsis thaliana. Plant Cell Reports 18: 20-24.

Sriraman, P., Silhavy, D., and Maliga, P. (1998) The phage-type PclpP-53 promoter comprises sequences downstream of the transcription initiation site. Nucleic Acids Res. 26: 4874-4879.

Chaudhuri, S. and Maliga, P. (1997) New insights into plastid RNA editing. Trends in Plant Science, 2: 5-6.

Hajdukiewicz, P., Allison, L.A., Maliga, P. (1997) The two plastid RNA polymerases encoded by the nuclear and plastid compartments transcribe distinct groups of genes in tobacco plastids. EMBO J. 16, 4041-4048.

Serino, G. and Maliga, P. (1997) A negative selection scheme based on the expression of cytosine deaminase in plastids. Plant J. 12: 697-701

Chaudhuri, S. and Maliga, P. (1996) Sequences directing C to U editing of plastid psbL mRNA are located within a 22 nucleotide segment spanning the editing site. EMBO J. 15, 5958-5964.

Allison, L.A., Simon, L.D. and Maliga, P. (1996) Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants. EMBO J. 15, 2802-2809.

Back to Main Page