All-All was created and is maintained by the Computational Biochemistry Research Group at the Eidgenossische Technische Hochschule in Zurich, Switzerland. The Waksman Student Scholars Program is in no way responsible for the All-All resource.
All-All can perform many different types of analysis on protein sequences. It is a powerful tool and many of its capabilities are beyond the scope of this brief reference. One of its most simple and potentially useful analysis options, however, may be the "MulAlignment: Multiple alignment" option. This option allows the user to align and compare multiple protein sequences. Output is submitted to the user via E-mail as plain text.
Using this analysis option is very simple. Once the user has accessed the All-All input form, he or she must first define an e-mail address that any results should be sent to. This is done in the text input box labeled, "The result should be mailed to: ". Next, the sequences that you wish to analyze should be defined in the text input field labeled "Sequences: ". Protein sequences can be defined in two ways. One is to input the single letter code for the amino acid sequences directly. The other, and perhaps easier, method is to submit the SwissProt accession numbers of the desired protein sequences in the following format:
Sample SwissProt Accession Numbers
P12067;,P00697;,P37156;
Individual accession numbers must be followed by a semi-colon and a comma. The last number must end with a semi-colon.
After entering the sequences that you wish to analyze, scroll down the input form until you see a check box labeled "MulAlignment: Multiple alignment." Check this option and click on the button that says "Submit to CBRG." You should receive data via e-mail within a short period of time.
You are encouraged to experiment with some of the other analysis options that All-All offers. However, if you wish to use some of the other analysis tools offered by All-All, you are likely to need an application that is capable of interpreting "postscript" files. This can be done either with a postscript enabled LaserWriter or with a shareware program called "Ghostscript" from Aladdin Software.
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