Natural diversity of CRISPR spacers of Thermus: evidence of local spacer acquisition and global spacer exchange
Lopatina A, Medvedeva S, Artamonova D, Kolesnik M, Sitnik V, Ispolatov Y, Severinov K. Natural diversity of CRISPR spacers of Thermus: evidence of local spacer acquisition and global spacer exchange Philosophical Transactions of the Royal Society B: Biological Sciences, 2019; 374 (1772): 20180092

Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation.
Musharova O, Sitnik V, Vlot M, Savitskaya E, Datsenko KA, Krivoy A, Fedorov I, Semenova E, Brouns SJJ, Severinov K. Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation. Mol Microbiol. 2019 Mar 15.

Cryo-EM structure and in vitro DNA packaging of a thermophilic virus with supersized T=7 capsids.
Bayfield OW, Klimuk E, Winkler DC, Hesketh EL, Chechik M, Cheng N, Dykeman EC, Minakhin L, Ranson NA, Severinov K, Steven AC, Antson AA. Cryo-EM structure and in vitro DNA packaging of a thermophilic virus with supersized T=7 capsids. Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3556-3561.

Architecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting a Ribosomally Synthesized Peptide into a DNA Gyrase Poison.
Ghilarov D, Stevenson CEM, Travin DY, Piskunova J, Serebryakova M, Maxwell A, Lawson DM, Severinov K. Architecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting a Ribosomally Synthesized Peptide into a DNA Gyrase Poison. Mol Cell. 2019 Feb 21;73(4):749-762.e5. Epub 2019 Jan 17.

Single-nucleotide-resolution mapping of DNA gyrase cleavage sites across the Escherichia coli genome.
Sutormin D, Rubanova N, Logacheva M, Ghilarov D, Severinov K. Single-nucleotide-resolution mapping of DNA gyrase cleavage sites across the Escherichia coli genome. Nucleic Acids Res. 2019 Feb 20.

Xenogeneic Regulation of the Bacterial Transcription Machinery.
Tabib-Salazar A, Mulvenna N, Severinov K, Matthews SJ, Wigneshweraraj S. Xenogeneic Regulation of the Bacterial Transcription Machinery. J Mol Biol. 2019 Feb 15. pii: S0022-2836(19)30085-3.

BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site.
Gordeeva J, Morozova N, Sierro N, Isaev A, Sinkunas T, Tsvetkova K, Matlashov M, Truncaite L, Morgan RD, Ivanov NV, Siksnys V, Zeng L, Severinov K. BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site. Nucleic Acids Res. 2019 Jan 10;47(1):253-265.

Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference.
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y. Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference. Cell. 2019 Jan 10;176(1-2):239-253.e16.

Effects of Population Dynamics on Establishment of a Restriction-Modification System in a Bacterial Host.
Graovac S, Rodic A, Djordjevic M, Severinov K, Djordjevic M. Effects of Population Dynamics on Establishment of a Restriction-Modification System in a Bacterial Host. Molecules. 2019 Jan 7;24(1). pii: E198.

Biosynthesis of the RiPP trojan horse nucleotide antibiotic microcin C is directed by the N-formyl of the peptide precursor.
Dong SH, Kulikovsky A, Zukher I, Estrada P, Dubiley S, Severinov K, Nair SK. Biosynthesis of the RiPP trojan horse nucleotide antibiotic microcin C is directed by the N-formyl of the peptide precursor. Chem Sci. 2018 Dec 26;10(8):2391-2395.

Avoidance of Trinucleotide Corresponding to Consensus Protospacer Adjacent Motif Controls the Efficiency of Prespacer Selection during Primed Adaptation.
Musharova O, Vyhovskyi D, Medvedeva S, Guzina J, Zhitnyuk Y, Djordjevic M, Severinov K, Savitskaya E. Avoidance of Trinucleotide Corresponding to Consensus Protospacer Adjacent Motif Controls the Efficiency of Prespacer Selection during Primed Adaptation. MBio. 2018 Dec 4;9(6). pii: e02169-18.

CRISPR-Cas molecular beacons as tool for studies of assembly of CRISPR-Cas effector complexes and their interactions with DNA.
Mekler V, Kuznedelov K, Minakhin L, Murugan K, Sashital DG, Severinov K. CRISPR-Cas molecular beacons as tool for studies of assembly of CRISPR-Cas effector complexes and their interactions with DNA. Methods Enzymol. 2019;616:337-363. Epub 2018 Dec 1.

Controller protein of restriction-modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock.
Klimuk E, Bogdanova E, Nagornykh M, Rodic A, Djordjevic M, Medvedeva S, Pavlova O, Severinov K. Controller protein of restriction-modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock. Nucleic Acids Res. 2018 Nov 16;46(20):10810-10826.

Ultrahigh-throughput functional profiling of microbiota communities.
Terekhov SS, Smirnov IV, Malakhova MV, Samoilov AE, Manolov AI, Nazarov AS, Danilov DV, Dubiley SA, Osterman IA, Rubtsova MP, Kostryukova ES, Ziganshin RH, Kornienko MA, Vanyushkina AA, Bukato ON, Ilina EN, Vlasov VV, Severinov KV, Gabibov AG, Altman S. Ultrahigh-throughput functional profiling of microbiota communities. Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9551-9556.

Defining the seed sequence of the Cas12b CRISPR-Cas effector complex.
Jain I, Minakhin L, Mekler V, Sitnik V, Rubanova N, Severinov K, Semenova E. Defining the seed sequence of the Cas12b CRISPR-Cas effector complex. RNA Biol. 2018 Aug 17:1-10.

New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas System.
Maikova A, Severinov K, Soutourina O. New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas System. Front Microbiol. 2018 Jul 31;9:1740.